Author Affiliations
Abstract
Grünberg Research Centre, College of Telecommunications and Information Engineering, Nanjing University of Posts and Telecommunications, Nanjing 210003, China
To date, fluorescence imaging systems have all relied on at least one beam splitter (BS) to ensure the separation of excitation light and fluorescence. Here, we reported multi-layer long pass filter integrated GaN LED. It is considered as the potential source for imaging systems. Experimental results indicate that the GaN LED shows blue emission peaked at 470.3 nm and can be used to excite dye materials. Integrating with a long pass filter (550 nm), the light source can be used to establish a real-time fluorescence detection for dyes that emit light above 550 nm. More interestingly, with this source, a real-time imaging system with signature words written with the dyes, such as ‘N J U P T’, can be converted into CCD images. This work may lead to a new strategy for integrating light sources and BS mirrors to build mini and smart fluorescence imaging systems.
GaN LED self-filtering illumination source fluorescence imaging Chinese Optics Letters
2023, 21(1): 011101
1 东北大学机械工程与自动化学院, 辽宁 沈阳 110819
2 沈阳精新再制造有限公司, 辽宁 沈阳 110142
利用激光熔覆技术对Ni204进行了单道正交试验, 采用激光共聚焦显微镜测量熔道几何尺寸及组织, 采用方差分析不同工艺参数对试样形貌以及组织的影响。结果表明, 功率对熔宽、熔深影响最显著; 扫描速度对熔高、宽高比(W/H)、润湿角影响最显著; 能量密度过小时, Ni204合金粉末在基体上没有形成熔池, 熔池边界处组织为柱状晶, 熔池内组织生长时间短且不易再结晶, 熔池内为枝晶。通过分析, 最优工艺参数组为Lp＝750 W, Pfr＝0.8 r/min, Ss＝480 mm/min与Lp＝900 W, Pfr＝1 r/min, Ss＝480 mm/min。可以在保证横向、纵向搭接的同时又不降低熔覆效率。
激光熔覆 正交实验 试样形貌 熔道组织 laser cladding orthogonal test sample morphology clad track microstructure
Author Affiliations
Abstract
Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics — Huazhong University of Science and Technology, Wuhan 430074, P. R. China
This paper proposes a method for predicting the reduced scattering coefficients of tissuesimulating phantoms or the desired amount of scatters for producing phantoms according to Mie scattering theory without measurements with other instruments. The concentration of the scatters TiO2 particles is determined according to Mie theory calculation and added to transparent host epoxy resin to produce phantoms with different reduced scattering coefficients. Black India Ink is added to alter the absorption coefficients of the phantoms. The reduced scattering coefficients of phantoms are measured with single integrating sphere system. The results show that the measurements are in direct proportion to the concentration of TiO2 and have identical with Mie theory calculation at multiple wavelengths. The method proposed can accurately determine the concentration of scatters in the phantoms to ensure the phantoms are qualified with desired reduced scattering coefficients at specified wavelength. This investigation should be possible to manufacture the phantom simply in reasonably accurate for evaluation of biomedical optical imaging systems.
Mie scattering theory integrating sphere optical imaging Journal of Innovative Optical Health Sciences
2010, 3(1): 53–59
Author Affiliations
Abstract
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
A fluorescence molecular tomography system for in vivo tumor imaging is developed using a 748-nm continuous wave diode laser as an excitation source. A high sensitivity cooled charge-coupled device (CCD) camera with excitation and emission filters is utilized to obtain the excitation and fluorescence images. The laser beam performs fast raster scanning using a dual-axis galvanometric scanner. The accuracy of the laser spot position at the source window is within +-200 \mum. Based on the phantom experimental results, the spatial resolution is less than 1.7 mm, and the relative quantitation error is about 10%. In vivo imaging of a tumor-bearing nude mouse tagged with near-infrared dye demonstrates the feasibility of the system.
荧光分子层析成像 小动物成像 170.6960 Tomography 170.6280 Spectroscopy, fluorescence and luminescence 170.0110 Imaging systems Chinese Optics Letters
2010, 8(11): 1075
